25175 only reduced og1rf  (ATCC)

Journal: bioRxiv

Article Title: Streptococcal natural products mediate interspecies competition with Gram-positive bacterial pathogens

doi: 10.1101/2025.07.31.667707

Figure Lengend Snippet: A. E. faecalis OG1RF CFU/mL in 1:10 co-culture with indicated Streptococcus spp. strain in BHI at 0, 2, 4, 6, and 24 h. For each culture, n = 2 technical replicates for each of n = 4 biological replicates. B. E. faecalis OG1RF CFU/mL in 1:10 co-culture with indicated S. mutans strain or S. sobrinus ATCC 33402 in BHI at 0, 2, 4, 6, and 24 h. For each culture, n = 2 technical replicates for each of n = 6-8 biological replicates. For A and B, two-way ANOVA was used for statistical analysis (ns, not significant; *, p = 0.0162; ***, p ≤ 0.0006; ****, p < 0.0001), each dot represents the mean of technical replicates, bars represent the mean of biological replicates, error bars represent standard errors of the mean, dashed lines indicate limit of detection (LOD), and data points at y = 0 indicate no CFUs were detected.

Article Snippet: While co-culture with UA159 reduced OG1RF CFU/mL starting at 4 h and by a maximum of 3.4 logs at 6 h compared to the highest CFU/mL at 2 h, co-culture with ATCC 25175 only reduced OG1RF CFU/mL by 1.6 logs at 6 h compared to 2 h and OG1RF viability recovered at 24 h ( ).

Techniques: Co-Culture Assay

Journal: bioRxiv

Article Title: Streptococcal natural products mediate interspecies competition with Gram-positive bacterial pathogens

doi: 10.1101/2025.07.31.667707

Figure Lengend Snippet: A. E. faecalis OG1RF CFU/mL in co-culture with indicated S. mutans UA159 erm R at indicated inoculum ratio of UA159 erm R to 1 part OG1RF in BHI at 0, 2, 4, 6, and 24 h. For each culture, n = 2 technical replicates for each of n = 4-6 biological replicates. Two-way ANOVA was used for statistical analysis (ns, not significant; *, p = 0.0149; **, p ≤ 0.0075; ****, p < 0.0001). B. CFU/mL of indicated E. faecalis clinical isolates in BHI monoculture at 0, 2, 4, 6, and 24 h. For each culture, n = 2 technical replicates for each of n = 1-3 biological replicates. C. CFU/mL of indicated Enterococcus spp. strains in BHI monoculture at 0, 2, 4, 6, and 24 h. For each culture, n = 2 technical replicates for each of n = 3 biological replicates. For A-C, each dot represents the mean of technical replicates, bars represent the mean of biological replicates, error bars represent standard errors of the mean, dashed lines indicate limit of detection (LOD).

Article Snippet: While co-culture with UA159 reduced OG1RF CFU/mL starting at 4 h and by a maximum of 3.4 logs at 6 h compared to the highest CFU/mL at 2 h, co-culture with ATCC 25175 only reduced OG1RF CFU/mL by 1.6 logs at 6 h compared to 2 h and OG1RF viability recovered at 24 h ( ).

Techniques: Co-Culture Assay

Journal: bioRxiv

Article Title: Streptococcal natural products mediate interspecies competition with Gram-positive bacterial pathogens

doi: 10.1101/2025.07.31.667707

Figure Lengend Snippet: A. pH of indicated overnight monoculture or co-culture. For each culture, n = 3 biological replicates. B. E. faecalis OG1RF CFU/mL cultured in BHI at indicated pH at 0, 1, 2, and 4 h. For each culture, n = 2 technical replicates for each of n = 4 biological replicates. C. E. faecalis OG1RF CFU/mL in co-culture with indicated S. mutans UA159 strain in BHI at 0, 2, 4, 6, and 24 h. For each culture, n = 2 technical replicates for each of n = 3-5 biological replicates. Two-way ANOVA was used for statistical analysis (ns, not significant). D. S. mutans UA159 erm R CFU/mL in 10:1 co-culture with E. faecalis OG1RF in BHI at 0, 2, 4, 6, and 24 h. For each culture, n = 2 technical replicates for each of n = 2-7 biological replicates. E. CFU/mL of E. faecalis OG1RF and OG1RF 24 h co-culture isolates in 1:10 co-culture with S. mutans UA159 erm R in BHI at 0, 2, 4, 6, and 24 h. For each culture, n = 2 technical replicates for each of n = 3 biological replicates. For A-E, each dot represents the mean of technical replicates, bars represent the mean of biological replicates, error bars represent standard errors of the mean, dashed lines indicate limit of detection (LOD), and data points at y = 0 indicate no CFUs were detected.

Article Snippet: While co-culture with UA159 reduced OG1RF CFU/mL starting at 4 h and by a maximum of 3.4 logs at 6 h compared to the highest CFU/mL at 2 h, co-culture with ATCC 25175 only reduced OG1RF CFU/mL by 1.6 logs at 6 h compared to 2 h and OG1RF viability recovered at 24 h ( ).

Techniques: Co-Culture Assay, Cell Culture

Journal: bioRxiv

Article Title: Streptococcal natural products mediate interspecies competition with Gram-positive bacterial pathogens

doi: 10.1101/2025.07.31.667707

Figure Lengend Snippet: A. CFU/mL of indicated E. faecalis clinical isolates in 1:10 co-culture with S. mutans UA159 erm R in BHI at 24 h (data is the same as shown in ). Representative gelatinase assay images are included on the x-axis for each E. faecalis clinical isolate. For each co-culture, n = 2 technical replicates for each of n = 3 biological replicates. For the gelatinase assays, n = 2 technical replicates per n = 2-5 biological replicates. Scale bar represents 5 mm. B. CFU/mL of indicated OG1RF strains in 1:10 co-culture with S. mutans UA159 erm R in BHI at 0, 2, 4, 6, and 24 h. For each culture, n = 2 technical replicates for each of n = 4 biological replicates. Two-way ANOVA was used for statistical analysis (**, p = 0.0015; ***, p = 0.0002; ****, p < 0.0001). C. CFU/mL of indicated E. faecalis clinical isolates in 1:10 co-culture with S. mutans ATCC 33535 in BHI at 24 h. For each co-culture, n = 2 technical replicates for each of n = 3 biological replicates. For A and C, upper dashed lines indicate the E. faecalis inoculum of 10 7 CFU/mL. For A, B, and C, each dot represents the mean of technical replicates, bars represent the mean of biological replicates, error bars represent standard errors of the mean, lower dashed lines indicate limit of detection (LOD), and data points at y = 0 indicate no CFUs were detected.

Article Snippet: While co-culture with UA159 reduced OG1RF CFU/mL starting at 4 h and by a maximum of 3.4 logs at 6 h compared to the highest CFU/mL at 2 h, co-culture with ATCC 25175 only reduced OG1RF CFU/mL by 1.6 logs at 6 h compared to 2 h and OG1RF viability recovered at 24 h ( ).

Techniques: Co-Culture Assay

Journal: bioRxiv

Article Title: Streptococcal natural products mediate interspecies competition with Gram-positive bacterial pathogens

doi: 10.1101/2025.07.31.667707

Figure Lengend Snippet: A. E. faecalis OG1RF CFU/mL in 1:10 co-culture with indicated S. mutans strain in BHI at 0, 2, 4, 6, and 24 h. For each culture, n = 2 technical replicates for each of n = 2-4 biological replicates. B. Genetic organization of the S. mutans UA159 mutanobactin biosynthetic gene cluster and location of the UA159B ISSmu1 insertion in mubD . C. E. faecalis OG1RF CFU/mL in 1:10 co-culture with indicated S. mutans UA159 strain in BHI ± 1% sucrose at 0 and 4 h. For each culture, n = 2 technical replicates for each of n = 3-7 biological replicates. D. CFU/mL of indicated strains in 1:10 co-culture with indicated S. mutans UA159 strain in BHI at 4 h. For each culture, n = 2 technical replicates for each of n = 3 biological replicates. For A, C, and D, two-way ANOVA was used for statistical analysis (ns, not significant; *, p = 0.0153; **, p ≤ 0.009; ***, p ≤ 0.0008; ****, p < 0.0001), each dot represents the mean of technical replicates, bars represent the mean of biological replicates, error bars represent standard errors of the mean, dashed lines indicate limit of detection (LOD), and data points at y = 0 indicate no CFUs were detected.

Article Snippet: While co-culture with UA159 reduced OG1RF CFU/mL starting at 4 h and by a maximum of 3.4 logs at 6 h compared to the highest CFU/mL at 2 h, co-culture with ATCC 25175 only reduced OG1RF CFU/mL by 1.6 logs at 6 h compared to 2 h and OG1RF viability recovered at 24 h ( ).

Techniques: Co-Culture Assay

Journal: bioRxiv

Article Title: Streptococcal natural products mediate interspecies competition with Gram-positive bacterial pathogens

doi: 10.1101/2025.07.31.667707

Figure Lengend Snippet: A. E. faecalis OG1RF CFU/mL in 1:10 co-culture with indicated S. mutans UA159 strain in BHI at 0, 2, 4, 6, and 24 h. For each culture, n = 2 technical replicates for each of n = 4-7 biological replicates. B. E. faecalis OG1RF CFU/mL in 1:10 co-culture with indicated S. mutans UA159 strain in BHI + 1% sucrose at 0, 2, 4, 6, and 24 h. For each culture, n = 2 technical replicates for each of n = 3 biological replicates. C. CFU/mL of indicated strain in monoculture in BHI at 0, 2, 4, 6, and 24 h. For each culture, n = 2 technical replicates for each of n = 2-3 biological replicates. D. CFU/mL of indicated strain in 1:10 co-culture with S. mutans UA159 erm R in BHI at 0, 2, 4, 6, and 24 h. For each culture, n = 2 technical replicates for each of n = 3 biological replicates. E. CFU/mL of indicated strain in 1:10 co-culture with S. mutans UA159 Δ mubD in BHI at 0, 2, 4, 6, and 24 h. F. CFU/mL of indicated strain in 1:10 co-culture with S. mutans UA159 Δ mubR in BHI at 0, 2, 4, 6, and 24 h. For each culture, n = 2 technical replicates for each of n = 3 biological replicates. For A, B, and D, two-way ANOVA was used for statistical analysis (*, p < 0.05; **, p = 0.002; ***, p ≤ 0.0008; ****, p < 0.0001).

Article Snippet: While co-culture with UA159 reduced OG1RF CFU/mL starting at 4 h and by a maximum of 3.4 logs at 6 h compared to the highest CFU/mL at 2 h, co-culture with ATCC 25175 only reduced OG1RF CFU/mL by 1.6 logs at 6 h compared to 2 h and OG1RF viability recovered at 24 h ( ).

Techniques: Co-Culture Assay

Journal: bioRxiv

Article Title: Streptococcal natural products mediate interspecies competition with Gram-positive bacterial pathogens

doi: 10.1101/2025.07.31.667707

Figure Lengend Snippet: A. CPRG hydrolysis of E. faecalis OG1RF at 24 h culture in TBS-D containing subinhibitory concentrations of indicated antibiotic. For each culture, n = 2 technical replicates for each of n = 5 biological replicates. B. CPRG hydrolysis of E. faecalis OG1RF at 24 h co-culture with indicated S. mutans UA159 strain in TBS-D. Representative images of CPRG assay cultures are included on the x-axis for each co-culture. For each culture, n = 2 technical replicates for each of n = 4 biological replicates. C. E. faecalis OG1RF CFU/mL grown in BHI containing 50% cell-free supernatant from indicated 4 h monoculture or co-culture. For each culture, n = 2 technical replicates for each of n = 3 biological replicates, dashed line indicates limit of detection (LOD), and data points at y = 0 indicate no CFUs were detected. For A-C, one-way ANOVA was used for statistical analysis (ns, not significant; **, p = 0.0053; ** p = 0.0096; ***, p = 0.0009; ****, p < 0.0001), each dot represents the mean of technical replicates, bars represent the mean of biological replicates, error bars represent standard errors of the mean.

Article Snippet: While co-culture with UA159 reduced OG1RF CFU/mL starting at 4 h and by a maximum of 3.4 logs at 6 h compared to the highest CFU/mL at 2 h, co-culture with ATCC 25175 only reduced OG1RF CFU/mL by 1.6 logs at 6 h compared to 2 h and OG1RF viability recovered at 24 h ( ).

Techniques: Co-Culture Assay

Journal: bioRxiv

Article Title: Streptococcal natural products mediate interspecies competition with Gram-positive bacterial pathogens

doi: 10.1101/2025.07.31.667707

Figure Lengend Snippet: A. Experimental set-ups for the co-culture Aclar biofilm assays for panels B and C and the pre-formed Aclar biofilm assays for panels D and E. For the co-culture biofilm assays, Aclar disks are submerged in media inoculated with both E. faecalis OG1RF and S. mutans UA159. For the pre-formed biofilm assays, Aclar disks are cultured with either OG1RF or UA159 for 24 h before being transferred to an OG1RF or UA159 mono-culture. B. E. faecalis OG1RF CFU per Aclar disk in co-culture with S. mutans UA159 erm R at indicated inoculum ratio of UA159 erm R to 1 part OG1RF in BHI at 4 and 24 h. For each co-culture, n = 2 technical replicates for each of n = 7-8 biological replicates. C. E. faecalis OG1RF CFU per Aclar disk in 1:10 co-culture with indicated S. mutans UA159 strain in BHI at 4 and 24 h. For each co-culture, n = 2 technical replicates for each of n = 3-4 biological replicates. D. E. faecalis OG1RF CFU per Aclar disk of OG1RF pre-formed Aclar biofilm in co-culture with indicated S. mutans UA159 strain in BHI at 4 and 24 h. For each co-culture, n = 2 technical replicates for each of n = 4 biological replicates. E. E. faecalis OG1RF CFU per Aclar disk in co-culture with pre-formed Aclar biofilm of indicated S. mutans UA159 strain in BHI + 1% sucrose at 4 and 24 h. For each co-culture, n = 2 technical replicates for each of n = 3-5 biological replicates. For B-D, two-way ANOVA was used for statistical analysis (ns, not significant; *, p = 0.0115; **, p = 0.0014; ***, p = 0.0005; ****, p < 0.0001), each dot represents the mean of technical replicates, bars represent the mean of biological replicates, error bars represent standard errors of the mean, dashed lines indicate limit of detection (LOD), and data points at y = 0 indicate no CFUs were detected.

Article Snippet: While co-culture with UA159 reduced OG1RF CFU/mL starting at 4 h and by a maximum of 3.4 logs at 6 h compared to the highest CFU/mL at 2 h, co-culture with ATCC 25175 only reduced OG1RF CFU/mL by 1.6 logs at 6 h compared to 2 h and OG1RF viability recovered at 24 h ( ).

Techniques: Co-Culture Assay, Cell Culture

Journal: bioRxiv

Article Title: Streptococcal natural products mediate interspecies competition with Gram-positive bacterial pathogens

doi: 10.1101/2025.07.31.667707

Figure Lengend Snippet: A. Planktonic E. faecalis OG1RF CFU/mL in co-culture with S. mutans UA159 erm R at indicated inoculum ratio of UA159 erm R to 1 part OG1RF in BHI at 4 and 24 h. For each co-culture, n = 2 technical replicates for each of n = 7-8 biological replicates. B. Planktonic E. faecalis OG1RF CFU/mL in 1:10 co-culture with indicated S. mutans UA159 strain in BHI at 4 and 24 h. For each co-culture, n = 2 technical replicates for each of n = 3-4 biological replicates. C. Planktonic E. faecalis OG1RF CFU/mL of OG1RF pre-formed Aclar biofilm in co-culture with indicated S. mutans UA159 strain in BHI at 4 and 24 h. For each co-culture, n = 2 technical replicates for each of n = 4 biological replicates. D. Planktonic E. faecalis OG1RF CFU/mL in co-culture with pre-formed Aclar biofilm of indicated S. mutans UA159 strain in BHI at 4 and 24 h. For each co-culture, n = 2 technical replicates for each of n = 3-5 biological replicates. For A-D, two-way ANOVA was used for statistical analysis (ns, not significant; *, p = 0.0374; **, p < 0.003; ***, p = 0.0002; ****, p < 0.0001), each dot represents the mean of technical replicates, bars represent the mean of biological replicates, error bars represent standard errors of the mean, dashed lines indicate limit of detection (LOD), and data points at y = 0 indicate no CFUs were detected.

Article Snippet: While co-culture with UA159 reduced OG1RF CFU/mL starting at 4 h and by a maximum of 3.4 logs at 6 h compared to the highest CFU/mL at 2 h, co-culture with ATCC 25175 only reduced OG1RF CFU/mL by 1.6 logs at 6 h compared to 2 h and OG1RF viability recovered at 24 h ( ).

Techniques: Co-Culture Assay